Reconstitution Guide
How to mix semaglutide powder?
Semaglutide Reconstitution Steps
- Clean your hands
- Collect all materials (show the semaglutide vial, bacteriostatic water vial, syringe with needle, alcohol wipe, sharps container for safe disposal of needles)
- Preparation 1. Remove caps from both vials and clean the top of the vials with an alcohol wipe. 2. Fix the needle to the syringe without letting it touch any other surface.
- Fill the syringe with bacteriostatic water 1. Hold the bacteriostatic water vial upside down and push the needle of the syringe into the rubber cap of the vial. Slowly retract the plunger on the syringe to the 1 ml line.
- Inject bacteriostatic water into the semaglutide powder vial 1. Insert the syringe with bacteriostatic water into the semaglutide vial 2. Push the plunger in slowly to inject all the water into the semaglutide vial 3. Pull the needle out once all the water has been injected
- Note: to inject more bacteriostatic water into your semaglutide (based on the dosing schedule), repeat steps Fill & Inject.
- Swirl the semaglutide vial gently to mix
How To Inject Semaglutide Injection (semaglutide reconstitution)?
The semaglutide STEP trial used the following doses. Here is how to create a solvent mix of the same semaglutide doses.
Using the 2mg semaglutide vial
- For 0.25 mg semaglutide dose: Add 2 mL bacteriostatic water to vial. Draw 0.25 mL semaglutide solution into syringe (you can do this 8 times)
- For 0.5 mg semaglutide dose: Add 2 mL bacteriostatic water to vial. Draw 0.5 mL semaglutide solution into syringe (you can do this 4 times)
- For 1 mg semaglutide dose: Add 1 mL bacteriostatic water to vial. Draw 0.5 mL semaglutide solution into syringe (you can do this 3 times)
- For 1.7 mg semaglutide dose: Add 1 mL bacteriostatic water to vial. Draw 1 mL semaglutide solution into syringe (you can do this 1 time).
Using the 5mg semaglutide vial
- For 0.5 mg semaglutide dose: Add 2 mL bacteriostatic water to vial. Draw 0.2 mL semaglutide solution into syringe (you can do this 10 times)
- For 1 mg semaglutide dose: Add 1 mL bacteriostatic water to vial. Draw 0.2 mL semaglutide solution into syringe (you can do this 5 times)
- For 1.7 mg semaglutide dose: Add 1 mL bacteriostatic water to vial. Draw 0.33 mL semaglutide solution into syringe (you can do this 3 times)
- For 2.4 mg semaglutide dose: Add 1 mL bacteriostatic water to vial. Draw 0.5 mL semaglutide solution into syringe (you can do this 2 times)
Semaglutide Doses
Reconstitution of 2 mg vial
| Dose | Volume of bacteriostatic water (mL)* | Volume of solution in the syringe (mL)** | Number of doses in one vial*** | Concentration per 1 mL |
|---|---|---|---|---|
| 0.25 mg | 2 mL | 0.25 mL | 8 | 1 mg/mL |
| 0.5 mg | 2 mL | 0.5 mL | 4 | 1 mg/mL |
| 1 mg | 1 mL | 0.5 mL | 2 | 2 mg/mL |
| 1.7 mg | 1 mL | 1 mL | 1 | 2 mg/mL |
Reconstitution of 5 mg vial
| Dose | Volume of bacteriostatic water (mL)* | Volume of solution in the syringe (mL)** | Number of doses in one vial*** | Concentration per 1 mL |
|---|---|---|---|---|
| 0.5 mg | 2 mL | 0.2 mL | 10 | 5 mg/mL |
| 1 mg | 1 mL | 0.2 mL | 5 | 2.5 mg/mL |
| 1.7 mg | 1 mL | 0.33 mL | 3 | 2.5 mg/mL |
| 2.4 mg | 1 mL | 0.5 mL | 2 | 2.5 mg/mL |
*This column tells you how much bacteriostatic water you should add to the vial.
**This column tells you how much of the produced solution you should draw up in the syringe so that the syringe contains the desired dose.
***This column tells how many times you can draw up the desired dose with the syringe per vial.
How to Store Reconstituted Semaglutide
Temperature
Semaglutide must be stored in the refrigerator to keep the solution stable. Ideally, the temperature should be within 36°F to 46°F (2°C to 8°C).
Labeling
Label each vial with the concentration of the solution, the date of mixing (reconstitution), and if being used in a laboratory by multiple users, you could add the initials of the person who mixed it (for traceability).
Aliquoting
Think about splitting the reconstituted peptide solution into smaller portions. This approach helps avoid multiple freeze-thaw cycles, which can reduce the likelihood of degradation.
Avoid Freeze-Thaw Cycles
Minimize the number of times you change the temperature of the semaglutide.
What To Know More About Semaglutide?
Have questions about semaglutide?
Go to our Semaglutide FAQ to learn more, or contact us.
